Mycobacterium Leprae Ziehl Neelsen
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Leprae from beg m.
Mycobacterium leprae ziehl neelsen. Leprae is not cultivable either in artificial culture media or in tissue culture. Ziehl neelsen stain for afb adapted from. Mycobacterium leprae in comparision to mycobacterium tuberculosis are much less acid and alcohol fast.
Mycobacterium does not bind readily to simple stains and therefore the use of heat along with carbol fuschin and phenol allows penetration through the bacterial cell wall for visualization. Principle of the ziehl neelsen staining the ziehl neelsen stain uses basic fuchsin and phenol compounds to stain the cell wall of mycobacterium species. Modified ziehl neelsen stain wade fite stain.
As the causative agents for leprosy mycobacterium leprae and nocardiasis nocardia asteroides are. The differentia tion is accomplished by the use of ziehl neelsens truants and bakers staining methods combined with treatment with py ridine. Zep raemurium is described.
Leprae and nontuberculous mycobacteria ntm. The only certain way to cultivate m. 1 bancroft stevens 2nd edition 1982 2 amer j clin pathol vol 21 1951 3 fremantle hospital department of histopathology western australia principle.
Acid fast organisms are those which are capable of retaining the primary stain when treated with an acid fastholding capacity. This procedure is used to stain mycobacterium tuberculosis and mycobacterium leprae. Other animals such as nine banded armadillo can also be used which is a natural host and reservoir of the pathogen.
Ziehl neelsen zn method of acid fast staining technique is used to stain mycobacterium species including m. Hence they get frequently decolorized by the standard ziehl neelsen technique. Leprae is by inoculating the specimens into fool pad of mice and keeping at 200c for 6 9 months.
The leprosy bacillis lipid envelope is much more affected by the fat solvents xylene traditionally used to dewax sections. Smegmatis an atypical mycobacteria a hamster lesion mycobacterium and m. Mycobacterium leprae are much less acid and alcohol fast as compared to mycobacterium tuberculosis.
Ziehlneelsen staining is a bacteriological stain used to identify acid fast organisms mainly mycobacteria. These bacteria are also called acid fast bacilli. They retain the dye when treated with acid which is because of the presence of mycolic acid in their cell wall.
They stain with carbol fuschin which is a red dye.
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