Lepra Bacilli Staining Procedure
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Leprae compared with myco.
Lepra bacilli staining procedure. Dissolve the stain in the alcohol then add the koh and water mixture and allow to sit overnight. Wash in tap water. Stain with carbol fuchsin at room temperature for.
6 177 showed that such material could be stained by oiling the sections and staining them with carbol fuchsin while they were in that state but the present authors have found this method cumbersome though. Brasileira leprologia 1938 v. Blot dry and wash in running tap water for 3 minutes.
Warm sections and de paraffinize in a mixture of two parts xyleneone part vegetable oil for 15 mins. Fite faraco staining protocol for leprosy bacilli 1. The lesser acid fastness of myco.
Ziehl neelsen zn method of acid fast staining technique is used to stain mycobacterium species including m. Bacillus fixatives lactobacillus casei leprosy microscopy electron staining and labeling substances. Repeat if any xyline oil remains on the section.
The stained smears are examined with a quality microscope using the oil immersion objective x100 to determine the total number of bacilli. Tuberculosis makes it difficult to stain appropriately in paraffin sections. Apply 5 7 drops of carbol fuchsin stain to thoroughly moistened filter paper.
The slide is intermittently heated from underneath using a spirit lamp until fumes arise kept for 5min. Filter on carbol fuchsin solution do not heat for 20 mins. Procedure preparation of smear cover a heat fixed dried smear with a small rectangle of filter paper.
The effect of fixatives on staining procedures for lepra bacilli in tissues. Wash in tap water. Repeat if any xylene oil remain on the section.
Microscopic examination of skin smears. Films of lymph exudate from the ulcerated nodule on the skin or non ulcerated nodule as well as from scrapings or secretions from nasal mucosa can be stained with ziehl neelsen method with 5 per cent sulphuric acid. Detection of acid fast bacilli afb in stained and acid washed smears examined microscopically may provide the initial bacteriologic evidence of the presence of mycobacteria in a clinical specimen.
Rinse in distilled water until slide clears. Blot dry and wash in water. Procedure of wade fite stain slightly warm the sections and deparaffinize in a mixture of peanut oil and xylene 12 two changes 6 minutes each.
Deparaffinize in xylenepeanut oil mixture 2 changes 10 minutes each. Blanco fl fite gl. Drain slides blot off excess oil.
The characteristic acid fast bacilli are present in the films. Acid alcohol until pale pink dip until stain stops running.
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